Cells have oligosaccharides displayed on their cell surface that are important for cell-cell recognition. Your friend discovered a transmembrane glycoprotein, GP1, on a pathogenic yeast cell that is recognized by human immune cells. He decides to purify large amounts of GP1 by expressing it in bacteria. To his purified protein he then adds a branched 14-sugar oligosaccharide to the asparagine of the only Asn-X-Ser sequence found on GP1 (Figure Q15-48). Unfortunately, immune cells do not seem to recognize this synthesized glycoprotein. Which of the following statements is a likely explanation for this problem?
(a) The oligosaccharide should have been added to the serine instead of the asparagine.
(b) The oligosaccharide should have been added one sugar at a time.
(c) The oligosaccharide needs to be further modified before it is mature.
(d) The oligosaccharide needs a disulfide bond.
Answer:
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